SimplyGreen qPCR Master Mix Kit provides a convenient, reliable, and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR Master Mix contains strategically -engineered, next-generation Taq Polymerase, DNA Polymerase, providing for rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, compared to wild-type Taq DNA polymerase. DNA Polymerase has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with DNA Polymerase can be used with TA cloning vectors. The kit contains dye comparable to SYBR Green™ and EvaGreen™.
Applications
- Gene expression (mRNA) analysis.
- Microarray validation.
Quality Control
The quality of the SimplyGreen qPCR Master Mix Kit, is tested on a lot-to-lot basis to ensure consistent product quality.
Required Materials
- Real-time PCR tubes
- Real-time PCR instrument
- RNase – free H2O