The Total RNA Isolation Kit provides a fast, simple, and cost-effective method for total RNA isolation from plant samples. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species, longer than 100 bases, to bind to the glass fiber matrix of the spin column. The isolated total RNA is suitable for a variety of routine applications including RT-PCR, Northern Blotting, cDNA Synthesis and Mapping. The entire procedure can be completed within 60 minutes.
Applications
- RT-PCR.
- Northern blotting.
- Real-time RT-PCR.
Quality Control
The quality of the Total RNA Isolation Kit (Plant) is tested on a lot-to-lot basis to ensure consistent product quality.