GeneDireX, Inc.

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Total RNA Isolation Kit (Blood/ Cultured Cell/ Fungus)

Size: 100 Reactions


Sample:
Whole blood (up to 300 μl)
Mammalian cells (up to 1 x 10⁷)
Bacterial cells (up to 1 x 10⁹)
Fungus Cells (up to 1 x 10⁸)
Yield:
Up to 30 μg
Format: Spin column
Operation time: 25-40 minutes
Elution volume: 50-200 μl


Features

  • Fast procedure and delivering high-quality total RNA.
  • Ready-to-use RNA for high performance in any downstream application.
  • Consistent RNA yield from a small amount of starting material.

Kit Contents

Contents Size
Buffer RL 110 ml
Buffer RA 45 ml
Buffer RO 25 ml
Buffer W1 45 ml
Buffer W2 (Add ethanol) 15 ml (60 ml)
Buffer RE 10 ml
DR Column 50 pieces x 2
Collection Tube 50 pieces x 2

NT$6,500

Clear

The Total RNA Isolation Kit provides a fast, simple, and cost-effective method for the isolation of total RNA from the whole blood, mammalian cells and bacterial cells. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species bases to bind to the glass fiber matrix of the spin column while contaminants pass through the column. Impurities are efficiently washed away, and the pure RNA is eluted with the RE Buffer without phenol extraction or alcohol precipitation. The RNA purified with the Total RNA Isolation Kit is suitable for a variety of routine applications, including the RT-PCR, cDNA synthesis, Northern Blotting, differential display, primer extension, and mRNA selection. The entire procedure can be completed within 25-40 minutes.



Application
Downstream molecular biology applications, including real-time RT-PCR, microarray analysis, next-generation sequencing (RNA-Seq), northern blotting, and cloning.


Quality Control
The quality of the Total RNA Isolation Kit (Blood/ Cultured Cell/ Fungus) is tested on a lot-to-lot basis to ensure consistent product quality.

Protocol

NA017-0100

SN017-0100

MSDS

NA017-0100_MSDS

SN017-0100_MSDS

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