UCMSC eXOrich medium is a serum-free and ready-to-use medium. The composition is optimized for the exosomes production from umbilical cord-derived mesenchymal stem cell (UCMSC). For exosomes production, we recommend that UCMSCs be cultivated in dishes or flasks until 90% confluence under regular culture conditions, and then remove the original culture medium and wash cells with the PBS and UCMSC eXOrich to remove the residual medium. Then the culture medium was replaced with UCMSC eXOrich and cultured for 3-4 days before collecting the conditioned medium for exosome purification.
Users can also replace the original basal medium with UCMSC eXOrich, and subculture UCMSC for at least one passage, and then perform the above steps to obtain a conditioned medium for exosome purification.
Using UCMSC eXOrich culture medium for exosome production, there is no need to add common nutritional supplements such as serum or human platelet lysate; however, users can decide to add appropriate nutritional supplements based on actual needs.
Nanoparticle Tracking Analysis: Size verification

Compared to conventional medium: 2X exosome harvest

Flow Cytometry: CD9, CD63, CD81 surface marker verified

Exosome Harvest Workflow by eXOrich Medium

FAQ
Q1. How do we produce exosomes?
ANS: For exosome purification, we recommend starting by preparing a conditioned medium after cell culture. The supernatant is collected from cultured cells, and large fragments of cell debris, waste products produced by cell metabolism, as well as impurities in the culture medium are removed through ultra-high-speed centrifugation or centrifugation filtration. Following this, exosomes can be concentrated using tangential flow filtration (TFF) or purified again through size exclusion chromatography (SEC).
Q2. How do we enrich exosome production?
ANS: Exosomes are known as cell-secreted natural nanovesicles, and the low-yield of exosome production from MSC-conditioned medium under conventional culture conditions limits their applications. The optimal cell culture medium can enhance exosome secretion from MSCs. Therefore, we have developed two exosome enrichment media. ADSC eXOrich medium is for exosome secretion from adipose-derived stem cells and UCMSC eXOrich medium is for exosome secretion from umbilical cord mesenchymal stem cells.
Q3. Is the supplement needed?
ANS: In general, commonly used supplements like serum or human platelet lysate contain exosomes. Therefore, we do not recommend using supplements in the preparation of conditioned medium for exosome isolation. When producing exosomes using ADSC eXOrich or UCMSC eXOrich medium, there is no need to add common nutritional supplements like serum or human platelet lysate. However, users can decide to add appropriate nutritional supplements based on their specific needs.