Vascular endothelial growth factor (VEGF) is an important signaling protein involved in vessel formation. VEGF mediates increased vascular permeability, induces vasculogenesis and endothelial cell production, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor.

Expression System
Escherichia coli

Sequence

MAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR with polyhistidine tag at the C-terminus.

Species
Human

Tag
polyhistidine tag at the C-terminus

Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.

Activity
Measure by its ability to induce HUVEC cells proliferation.
The ED₅₀ for this effect is <5 ng/mL.
The specific activity of recombinant human VEGF165 is approximately >1.4 x 10⁶ IU/mg.

Purity
>98% as determined by SDS-PAGE. Ni-NTA chromatography

Formulation

The protein was lyophilized from a solution containing 1X PBS, pH 8.0.

Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 minutes to ensure sufficient re-dissolved.

SDS-PAGE analysis of recombinant human VEGF165