| Cat. No. MB702-0100 | Size: 100ul |
| Cat. No. SM702-0100 | Size: 100ul |
| Store at -20°C | Conc.: 50uM |
| Description Random Hexamers are short oligodeoxyribonucleotides of random sequences [d(N)6] that anneal to random complementary sites on a target DNA or RNA and serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase. During cDNA generation, random priming gives random coverage to all regions of the RNA to generate a cDNA pool containing various lengths of cDNA. Random priming is incapable of distinguishing between the mRNA and other RNA species present in the reaction. |
| Protocol We recommend using 1 μl of Random Hexamers primer per 20 μl reaction volume. |
| Quality control This product is qualified in a cDNA reaction. |
| Applications ◆ cDNA synthesis using a Reverse Transcriptase with RNA templates ◆ DNA synthesis using the Klenow fragment with DNA templates ◆ DNA probe synthesis for use in Southern, Northern, and in situ hybridization applications |
| Notes For research use only. |
