Random Hexamers primer

Cat. No. MB702-0100Size: 100ul
Cat. No. SM702-0100Size: 100ul
Store at -20°CConc.: 50uM

Description
Random Hexamers are short oligodeoxyribonucleotides of random sequences [d(N)6] that anneal to random complementary sites on a target DNA or RNA and serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase.
During cDNA generation, random priming gives random coverage to all regions of the RNA to generate a cDNA pool containing various lengths of cDNA. Random priming is incapable of distinguishing between the mRNA and other RNA species present in the reaction.
Protocol
We recommend using 1 μl of Random Hexamers primer per 20 μl reaction volume.
Quality control
This product is qualified in a cDNA reaction.
Applications
◆ cDNA synthesis using a Reverse Transcriptase with RNA templates
◆ DNA synthesis using the Klenow fragment with DNA templates
◆ DNA probe synthesis for use in Southern, Northern, and in situ hybridization applications
Notes
For research use only.

Download
MB702-0100ProtocolMSDS
SM702-0100ProtocolMSDS